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HX Design Challenge

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Heat Exchanger Design Challenge The objective of this project was to design a shell-and-tube heat exchanger that meets the heat requirement of 170,453 𝑤𝑎𝑡𝑡𝑠, and with consideration that the optimum overall heattransfer coefficient is 285 𝑊/𝑚2⋅˚𝐶. The shell-and-tube heat exchanger is used to heat benzene by having hot water flow through a single-pass shell. A counter-current flow was used because it is more efficient. The pressure-drop considerations of the benzene in the tubes are fixed at a maximum velocity of 1.2 m/s. Due to space limitations, the length of the heat exchanger must not exceed 6 m. The tubes used for the design are ¾ in OD 17 BWG tubes. Segmental baffles, or 25% cut baffles, were included to ensure that the water will flow across the tubes, thus enhancing the heat transfer. The target overall heat-transfer coefficient, 𝑈, is 10-25% higher than the optimal 𝑈 to ensure that the heat requirement of 170,453 𝑤𝑎𝑡𝑡𝑠 is met. Copper tubes were u...

Co-expressing Disulfide Bond Proteins

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Molecular chaperones and protein folding factors of bacterial periplasmic space play important roles in assisting disulfide bond formation and proper protein folding. In this study, effects of disulfide bond protein (Dsb) families were investigated on assembly of 3F3 Fab, an antibody inhibitor targeting matrix metalloproteinase-14 (MMP-14). By optimizing DsbA/C co-expression, promoter for 3F3 Fab, host strains, and culture media and conditions, a high yield of 30-mg purified 3F3 Fab per liter culture was achieved. Produced 3F3 Fab exhibited binding affinity of 34 nM and inhibition potency of 970 nM. This established method of DsbA/C co-expression can be applied to produce other important disulfide bond-dependent recombinant proteins in E. coli periplasm. find the article here: https://www.ncbi.nlm.nih.gov/pubmed/?term=Kruchowy

CADing Intro

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Check out dozens of my free CAD models on Onshape ! Link to this model here!
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